A method for rapid regulation of protein expression in Trypanosoma cruzi

Analysis of gene function in Trypanosoma cruzi is limited due to the absence of rapid, simple and reversible genetic tools to regulate gene and corresponding protein expression. We have designed a modified pTREX vector which uses an N-terminal fusion of a ligand-controlled destabilisation domain (ddFKBP) to a gene/protein of interest. This vector allows rapid and reversible protein expression and efficient functional analysis of proteins in different T. cruzi life cycle stages.

Graphical abstractFigure optionsDownload full-size imageDownload high-quality image (46 K)Download as PowerPoint slideHighlights► Regulation of protein expression in Trypanosoma cruzi. ► Modified pTREX vector with a ligand-controlled destabilisation domain. ► Rapid, inducible and reversible. ► Suitable for intracellular form study.