Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
2436255 | International Journal for Parasitology | 2012 | 8 Pages |
The single-celled parasite, Entamoeba histolytica, is an enteric pathogen that ingests bacteria and host cells. Inhibition of phagocytosis renders the parasite avirulent. The ligand/receptor interactions that allow E. histolytica to phagocytose are not well understood. We hypothesised that E. histolytica trophozoites might accomplish ingestion through the utilisation of a scavenger receptor for cholesterol. Here we show that acetylated low density lipoprotein cholesterol was phagocytosed by amoebae via receptor-mediated mechanisms. Acetylated low density lipoprotein cholesterol competitively inhibited by 31 ± 1.3% (P < 0.005) the ingestion of Escherichia coli, but not erythrocytes and Jurkat T lymphocytes, suggesting a partially redundant phagocytic pathway for E. coli and cholesterol. Inducible expression of a signalling-dead dominant-negative version of E. histolytica transmembrane kinase 39 inhibited ingestion of E. coli by 55 ± 3% (P < 0.005) but not LDL particles. We concluded that ingestion of E. coli was regulated by TMK39 and partially shared the acetylated low density lipoprotein cholesterol uptake pathway.
Graphical abstractFigure optionsDownload full-size imageDownload high-quality image (31 K)Download as PowerPoint slideHighlights► Entamoeba histolytica ingests low density lipoprotein (LDL) particles in a saturable manner. ► LDL particles are a competitive ligand for the ingestion of Escherichia coli bacteria. ► The adherence and ingestion of E. coli bacteria is regulated by transmembrane kinase 39.