Giardia lamblia Nek1 and Nek2 kinases affect mitosis and excystation

The NIMA-related serine/threonine kinases (Neks) function in the cell cycle and regulate ciliary and flagellar length. The Giardia lamblia genome encodes 198 Neks, of which 56 are predicted to be active. Here we believe that we report the first functional analysis of two G. lamblia Neks. The GlNek1 and GlNek2 kinase domains share 57% and 43% identity to the kinase domains of human Nek1 and Nek2, respectively. Both GlNeks are active in vitro, have dynamic relocalisation during the cell cycle, and are expressed throughout the life cycle, with GlNek1 being upregulated in cysts. Over-expression of inactive GlNek1 delays disassembly of the parental attachment disc and cytokinesis, whilst over-expression of either wild type GlNek1 or inactive mutant GlNek2 inhibits excystation.

Graphical abstractFigure optionsDownload full-size imageDownload high-quality image (40 K)Download as PowerPoint slideHighlights► The first report of functional analysis of two Giardia lamblia Nek kinases. ► Both GlNeks have dynamic re-localisation during the cell cycle. ► Over-expression of inactive Nek1 delays parental disc disassembly during the cell cycle. ► Over-expression of wild type Nek1 or inactive Nek2 inhibits excystation.