Challenges in environmental detection of human viral pathogens

There is substantial potential for human exposure to viruses in environmental matrixes. Identification of virally contaminated environmental reservoirs requires assays with sufficient sensitivity to detect low copy numbers of viral targets. However, low detection sensitivity frequently requires sample concentration during which inhibitors to downstream assays co-isolate with desired target. Conventional detection assays (e.g., cell culture, polymerase chain reaction) require a priori selection of appropriate cell lines or primers and probes based on the viruses anticipated to be present in the sample. This can underestimate exposure risks by excluding unidentified or unknown virus. Emerging methods including nonspecific adsorption/elution, filtration, and total nucleic acid sequencing, that are capable of concentrating, purifying, and detecting total virus and/or total virus nucleic acid will aid in estimates of exposure risk, source tracking, intervention efficacy, and evaluation of virus fate and transport. Development and implementation of novel virus detection techniques must integrate quality assurance guidelines to validate results and provide opportunities for interstudy comparison.

► Challenges in virus detection include assay sensitivity and sample inhibition. ► Current viral techniques requiring a priori identification limit effectiveness. ► Emerging sequence-independent viral assays show promise. ► Standardized quality assurance and control for environmental viral detection are needed.