Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
2495562 | Pharmaceutical Methods | 2012 | 5 Pages |
Abstract
Aim and Backrgound: A stability-indicating LC method was developed for the simultaneous determination of Ibuprofen and Famotidine in pharmaceutical dosage forms. Materials and Methods: The chromatographic separation was achieved on Acquity UPLC BEH C-18,50 mm à 2.1 mm and 1.7 μm column with gradient elution. The mobile phase A contains a mixture of 50 mM sodium acetate buffer (pH 5.5): methanol (85:15, v/v), and the mobile phase B contains a mixture of 50 mM sodium acetate buffer (pH 5.5): methanol (25:75, v/v). The flow rate was 0.3 mL min- 1, and the detection wavelength was 260 nm. Results: The limit of detection for Ibuprofen and Famotidine was 1.6 and 1.2 μg mL- 1, respectively. The limit of quantification (LOQ) for Ibuprofen and Famotidine was 5.1 and 4.3 μg mL- 1, respectively. Conclusion: This method was validated for accuracy, precision, and linearity. The method was also found to be stability indicating.
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Authors
Yarram Ramakoti Reddy, Kakumani Kishore Kumar, K. Mukkanti, M.R.P. Reddy,