Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
2495615 | Pharmaceutical Methods | 2011 | 4 Pages |
Abstract
Aim and Objectives: To develop a simple sensitive cost-efiective and reproducible UVspectrophotometric method and validate for the estimation of disodium edetate in topical gel formulations. Materials and Methods: Solution of disodium edetate reacts with ferric chloride to form complex in 0.1 N HC1 giving λmax at 270 nm. Beer's law was obeyed in the concentration range of 5-50 μg/mL (r2 = 0.9997). Results: The limit of detection and limit of quantitation were found to be 1.190 and 3.608 μg/mL respectively. The results show that the procedure is accurate precise and reproducible (relative standard deviation < 1%) while being simple and less time consuming. Conclusions: The study concluded that the UV-spectrophotometric method could be used for the quantification of disodium edetate in pure form as well as in pharmaceutical formulations.
Keywords
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Authors
Muralidharan Selvadurai, Subramaniya Nainar Meyyanathan,