Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
2495665 | Pharmaceutical Methods | 2011 | 5 Pages |
Abstract
Introduction: The present work aimed to develop and validate spectrophotometric methods for simultaneous estimation of rabeprazole sodium and aceclofenac in a pure and capsule dosage form. Materials and Methods: Method 1 is based on solving a simultaneous equation. Absorbances of rabeprazole sodium and aceclofenac were measured at their respective absorbance maximas (λmax ) of 283 and 276 nm. Method 2 is the Q-analysis or absorption ratio method. Absorbances were measured at 256 nm (isosbestic point) and 276 nm (λmax of aceclofenac). Methods are validated according to ICH guidelines. Results: A linearity range for rabeprazole sodium and aceclofenac is 10-60 μg/ml at respective selected wavelengths. The coefficient of correlation for rabeprazole at 283 nm and for aceclofenac at 276 nm is 0.9981 and 0.9997, respectively. A percentage estimation of rabeprazole sodium and aceclofenac from the capsule dosage form by method 1 is 100.22 and 99.96 and by method 2 is 99.99 and 100.05, respectively, with a standard deviation less than 2. Conclusion: The proposed methods are simple, rapid, and validated and can be used successfully for routine simultaneous estimation of rabeprazole sodium and aceclofenac in a pure and capsule dosage form.
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Authors
Ramesh L. Sawant, Sanket D. Hadawale, Ganesh K. Dhikale, Charusheela A. Bansode, Pravin S. Tajane,