Acetogenins from Annona muricata

Silica gel chromatography of the dichloromethane extract of the seeds of Annona muricata afforded annoreticuin-9-one (1), while the flesh of the fruit yielded cis-annoreticuin (2) and sabadelin (3). The structures of 1-3 were elucidated by extensive 1 D and 2D NMR spectroscopy and confirmed by mass spectrometry. Acetogenins 1 and 2 were first isolated from A. reticulata and A. montana, respectively. Acetogenin 1 was reported to exhibit cytotoxic activities against the human pancreatic tumor cell line (PACA-2), human prostate adenocarcinoma (PC-3) and human lung carcinoma (A-549), while 2 was reported to exhibit cytotoxicity against human hepatoma carcinoma cell line (Hep G2).