HPTLC method Development & Validation for quantification of Markers of Dhatrinisha churna

Introduction: Dhatrinisha churna has been traditionally used in the Ayurvedic system of medicine and by traditional medical practices of India to treat hyperlipidemia. A sensitive, reliable, selective, precise and accurate densitometric High Performance Thin Layer Chromatography (HPTLC) fingerprinting method has been developed for the simultaneous determination and quantification of Curcumin and Ellagic acid in Dhatrinisha churna. Method: Quantification of Curcumin and Ellagic acid were done by developed densitometric HPTLC method. Validation of method performs in order to demonstrate its selectivity, accuracy, precision, repeatability and recovery study. Result: All calibration curves showed good linear correlation coefficients (r2>0.997) within the tested ranges. The chromatogram of Dhatrinisha churna was quantified with respect to Curcumin (1.072% w/w) and Ellagic acid (0.867% w/w). Intra-and inter-day RSDs of retention times and peak areas were less than 1.92%. The recoveries were between 96.60 and 101.40%. Conclusion: A method has been developed for the simultaneous quantification of two markers in Dhatrinisha churna. The proposed HPTLC method was found to be simple, précised and accurate and can be used for the quality control of the raw materials as well as formulations.