Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
2496057 | Pharmacognosy Journal | 2010 | 7 Pages |
Abstract
Treatment of cell suspension cultures of Linum flavum L. with Na2EDTA reduced the coniferin and enhanced the 6-methoxypodophyllotoxin (6-MPT) production in a concentration-dependent way, in a range of 0.1-5 mM. On day 14 after treatment with Na2EDTA , an inhibition of the coniferin production up to 88% was found. The maximum enhancement of the 6-MPT production was 400% on day 7 after treatment with 5 mM Na2EDTA . The reduction in coniferin accumulation in the suspension cultures correlated with and inhibition of coniferyl alcohol glucosyltransferase (CAGT ) activity as determined in cell homogenates. On day 14 after treatment with 2 and 5 mM Na2EDTA , the CAGT activity was inhibited up to > 89 %. The inhibitory effect of Na2EDTA on CAGT was also shown in a partially purified enzyme preparation. Several metal ions and the elicitors nigeran and salicylic acid had no significant effect on the production of coniferin and 6-MPT.
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Authors
Elfahmi Elfahmi, Komar Ruslan Wirasutisna, Sieb Batterman, Albert Koulman, Oliver Kayser, Herman J. Woerdenbag, Wim J. Quax,