| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 2496588 | Phytomedicine | 2014 | 5 Pages |
Abstract
The role of RecA protein in bacterial resistance to antibiotics makes this protein attractive from a pharmacological point of view. In this study we demonstrate that curcumin is able to inhibit the SOS response in Escherichia coli induced by levofloxacin. The blaTEM-1 gene has been placed under the control of the LexA-binding box and used as reporter gene. The expression of TEM-1 β-lactamase enzyme was increased in the presence of ssDNA induced by levofloxacin, while, the presence of curcumin at 8 μg/ml, reduced dramatically the expression of the reporter gene. Moreover a simple microplate assay suitable for high-throughput screening has been developed.
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Authors
Pierangelo Bellio, Fabrizia Brisdelli, Mariagrazia Perilli, Alessia Sabatini, Carlo Bottoni, Bernardetta Segatore, Domenico Setacci, Gianfranco Amicosante, Giuseppe Celenza,