| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 2511295 | Antiviral Research | 2008 | 8 Pages |
Abstract
Gene-based delivery of recombinant antibody genes is a promising therapeutic strategy offering numerous advantages including sustained antibody levels, better safety profile and lower production cost. Here we describe generation of a recombinant antibody Fc-9E2 comprising a fusion protein between human Fc of IgG1 and a single-chain Fv derived from a hybridoma 9E2 secreting a mAb neutralizing West Nile virus (WNV). Fc-9E2 was shown to retain parental mAb's specificity and WNV-neutralizing capacity. Adenovirus-mediated in vivo delivery of the antibody gene resulted in sustained Fc-9E2 serum levels leading to abrogation of lethal WNV infection in an animal model.
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Immunology and Microbiology
Virology
Authors
Alexander Pereboev, Viktoriya Borisevich, George Tsuladze, Mikhail Shakhmatov, Deborah Hudman, Elena Kazachinskaia, Ivan Razumov, Viktor Svyatchenko, Valery Loktev, Vladimir Yamshchikov,