Pteridic acid hydrate and pteridic acid C produced by StreStreptomyces pseudoverticillus YN17707 induce cell cycle arrest

The present study aimed at identifying cell cycle inhibitors from the fermentation broth of Streptomyces pseudoverticillus YN17707. Activity-guided isolation was performed on tsFT210 cells. Compounds were isolated through various chromatographic methods and elucidated by spectroscopic analyses. Flow cytometry was used to evaluate the cell cycle inhibitory activities of the fractions and compounds. Two compounds were obtained and identified as pteridic acid hydrate (1) and pteridic acid C (2), which arrested the tsFT210 cells at the G0/G1 phase with the MIC values being 32.8 and 68.9 μmol·L−1, respectively. These results provide a basis for future development of Compounds 1 and 2 as novel cell cycle inhibitors for cancer therapy.

Two new spirocyclic polyketides, pteridic acid hydrate (1) and pteridic acid C (2), were obtained from the fermentation broth of Streptomyces pseudoverticillus YN17707 by activity-guided procedures. They arrested the cell cycle of tsFT210 cells at the G0/G1 phase with the MIC values being 32.8 μmol·L−1 and 68.9 μmol·L−1, respectively. These results provide a basis for future development of compounds 1 and 2 as novel cell cycle inhibitors for cancer therapy.Figure optionsDownload full-size imageDownload as PowerPoint slide