Effects of Danshensu on the Incidence of Ischemia-reperfusion Induced Arrhythmia in Hypertrophy Rat Heart

ABSTRACTAIMTo study the effects of Danshensu on the incidence of ischemia reperfusion (I/R) induced arrhythmia in hypertrophy rat and to explore its relationship with NO.METHODSL-thyroxine (2 mg·kg−1·d−1) po 10 d was used to establish myocardial hypertrophy model; rats were administered with either Danshensu or Valsartan from the 4th day to the 10th day. Ischemia and reperfusion arrhythmia was produced by ligating 20 min and loosing the coronary artery in Langendorff isolated rat heart; ECG was recorded on VT and VF incidence rate, onset time and duration time. Blood serums NO contents and NOS activity were determined and heart weight index was measured.RESULTSCompared with the control group, VF incidence of I/R episode was increased in the hypertrophy group; serum NO content was significantly reduced and eNOS activity was significantly dropped in the hypertrophy group (P<0.05). Danshensu and Valsartan pretreatment groups can effectively inhibit I/R arrhythmia, ventricular arrhythmia incidence rate, onset time and duration time of VT and VT were lower than in hypertrophy groups; Danshensu and Valsartan were effective in preventing hypertrophy progression in rats; serum NO content and eNOS activity were significantly enhanced toward normal range.CONCLUSIONDanshensu has shown a significant cardioprotection against I/R injury in hypertrophy rat heart and the cardioprotection of Danshensu is partly mediated by protecting of eNOS activity and increasing NO content, the effects are similar to those of Valsartan.