Triterpene Glycosides from Sea Cucumber Holothuria leucospilota

AimTo find new bioactive triterpene glycosides from the sea cucumber Holothuria leucospilota.MethodsColumn chromatography on silica gel and ODS RP-C18 and HPLC were used for the isolation and purification of triterpene glycosides, and their structures were elucidated on the basis of spectral data and chemical evidence.ResultsSeven triterpene glycosides were isolated from the sea cucumber H. leucospilota, and identified as leucospilotaside A (1), leucospilotaside B (2), leucospilotaside C (3), holothurin B (4), holothurin B2 (5), echinoside B (6), and holothurin A (7).ConclusionCompound 2 is a new triterpene glycoside, and compounds 1, 3, 5 and 6 have been isolated from this sea cucumber for the first time.