Neuroprotective Effects of Sinapine on PC12 Cells Apoptosis Induced by Sodium Dithionite

ABSTRACTAIMTo evaluate the effects of sinapine on sodium dithionite (Na2S2O4) induced cytotoxicity in differentiated rat pheochromocytoma (PC12) cells.METHODSPC12 cells hypoxia damage model was established by exposure to Na2S2O4, and the protective effect of sinapine was assayed by 3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide (MTT), lactate dehydrogenase(LDH), malondialdehyde (MDA) and flow cytometric (FCM) for apoptosis rate and mitochondrial transmembrane potential.RESULTSSinapine increased cell viability and showed its significant effect on protecting PC12 cells against Na2S2O4-induced apoptosis and the disruption of mitochondrial transmembrane potential by flow cytometric (FCM) analysis. In addition, sinapine also markedly attenuated the leakage of LDH and the lipid peroxidation products malondialdehyde (MDA) of Na2S2O4-induced in PC12 cells.CONCLUSIONIt suggests that sinapine may be a candidate chemical for the treatment of hypoxia neurodegenerative disease thro ugh anti-oxidation and anti-apoptosis.