Isolation and Standardization of Various Phytochemical Constituents from Methanolic Extracts of Fruit Rinds of Punica granatum

AimThe present study was undertaken to isolate and standardize the various active phytochemical constituents present in the fruit rinds of Punica granatum.MethodsFruit rinds of Punica granatum were dried and extracted with methanol in a static extractor; the percentage yield of the methanolic extract (MEPG) was found to be 26% the methanolic extract was partitioned using n-butanol, ethyl acetate and water; the percentage yield of the fractions were found to be 17.16%, 26.88% and 47.72% respectively. HPLC was carried out for all the fractions and the markers corresponding to the major peaks in HPLC were isolated using column chromatography. The isolated markers were characterized using UV, IR, Mass and NMR spectroscopy.Results & ConclusionTotally four compounds were isolated and identified as punicalin, punicalagin, gallic acid and ellagic acid based on UV, IR, Mass and NMR data, they were standardized by HPLC.