Intravital microscopy: new insights into cellular interactions

Inflammation is the body's way of combating invading pathogens or noxious stimuli. Under normal conditions, the complex host response of rubor, dolor, calor, tumor, and functio laesa is essential for survival and the return to homeostasis. However, unregulated inflammation is all too often observed in diseases such as rheumatoid arthritis, stroke, and cancer.The host inflammatory response is governed by a number of tightly regulated processes that enable cellular trafficking to occur at the sites of damage to ultimately ensure the resolution of inflammation. Intravital microscopy (IVM) provides quantitative, qualitative, and dynamic insights into cell biology and these cellular interactions.This review highlights the pros and cons of this specialized technique and how it has evolved to help understand the physiology and pathophysiology of inflammatory events in a number of different disease states, leading to a number of potential therapeutic targets for drug discovery.

► A brief overview of inflammation and leukocyte trafficking. ► Intravital microscopy and how it has evolved — with particular reference to fluorescence microscopy. ► The use of intravital microscopy in disease.