Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
2534893 | European Journal of Pharmacology | 2008 | 6 Pages |
Abstract
With human neuropeptide Y Y2 receptor expressed in the Chinese hamster ovary (CHO) cells, the Asp35Ala mutation, and especially the change of Pro34Asp35 to Ala34Ala35, decrease the compartmentalization and strongly accelerate internalization of the receptor. These changes are not associated with alterations in agonist affinity, G-protein interaction, dimerization, or level of expression of the mutated receptors relative to the wildtype receptor. The proline-flanked aspartate in the N-terminal extracellular segment of the neuropeptide Y Y2 receptor thus apparently has a large role in anchoring and compartmentalization of the receptor. However, the Pro34Ala mutation does not significantly affect the embedding and cycling of the receptor.
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Authors
Steven L. Parker, Michael S. Parker, Ying Y. Wong, Renu Sah, Ambikaipakan Balasubramaniam, Floyd Sallee,