Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
2540266 | International Immunopharmacology | 2016 | 6 Pages |
•We examined functions of tumor infiltrating T cells in osteosarcoma.•High frequencies of TIM-3+ and TIM-3+ PD-1+ T cells were found in tumor tissues.•TIM-3+ and TIM-3+ PD-1+ T cells showed weak proliferation and cytokine responses.•TIM-3+ PD-1+ T cells in tumor tissues were correlated with IL-10 and M2-type TAM.•Depletion of M2-type TAM improved T cell proliferation and cytokine production.
Osteosarcoma is one of the most common childhood cancers with high numbers of cancer-related deaths. Progress in conventional therapies is showing limited improvement. An adaptive T cell-based immunotherapy represents a promising new therapeutic option, but to improve its efficacy, regulatory mechanisms in osteosarcoma need further elucidation. Here, to evaluate the regulatory effect of tumor microenvironment of T cells in osteosarcoma, we examined the peripheral blood (PB) and tumor infiltrating (TI) T cells, and their correlations with PB and tumor immune characteristics. We found that TI T cells contained significantly higher levels of TIM-3+ PD-1− and TIM-3+ PD-1+ cells than their PB counterparts. Similar to that in chronic HIV and HCV infections, these TIM-3+ PD-1− and TIM-3+ PD-1+ T cells presented reduced proliferation and proinflammatory cytokine secretion in response to stimulation. Presence of M2-type (CD163+) macrophages exacerbated T cell immunosuppression, since frequencies of CD163+ tumor-associated macrophages were directly correlated with the frequencies of suppressed TIM-3+ PD-1+ T cells. Moreover, depletion of CD163+ macrophages significantly improved T cell proliferation and proinflammatory cytokine production. Overall, our data presented an intratumoral T cell-specific immunosuppression that was amplified by M2-type tumor-associated macrophages.