Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
2552842 | Life Sciences | 2008 | 7 Pages |
To elucidate the pharmacological profile of P2X receptors and the probable expression of P2Y receptors in otic ganglion neurons from 17-day-old rats, single neurons were enzymatically isolated and maintained in tissue culture for up to 30 h. Whole-cell voltage-clamp recording was carried out at a holding potential of − 60 mV. Most otic ganglion neurons responded to adenosine 5′-triphosphate (ATP), 2-methylthio ATP (2-MeSATP) and α,β-methylene ATP (αβ-meATP) with sustained currents and EC50 values of 19 µM, 47 µM and 94 µM, respectively. 2′,3′-O-trinitrophenyl-ATP (TNP-ATP) inhibited the response to αβ-meATP and ATP with an IC50 values of 3.9 nM and 18.3 nM, respectively, which was closed to that observed in nodose neurons. The response to ATP was antagonized by suramin and cibacron blue. The dose–response curve of suramin against ATP response at a pH of 6.5 was shifted to the left compared to that at a pH of 7.4. Diinosine pentaphosphate (Ip5I), which blocks P2X3, but not P2X2/3-mediated responses, had no effect on the currents evoked by ATP or αβ-meATP. In some neurons, uridine 5′-triphosphate (UTP) induced a tiny, but long-lasting current with a mean amplitude of 0.034 ± 0.011 nA. Reverse transcriptase-polymerase chain reaction (RT-PCR) confirmed the expression of mRNAs for P2X2, P2X3, P2X4, P2X6 and P2X7, but not for P2X1 and P2X5 receptors in otic ganglion. In conclusion, in rat otic ganglion neurons, P2X2/3 heteromultimer receptors dominate, but P2X7 and P2Y2 or P2Y4 receptors also play roles.