| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 2553774 | Life Sciences | 2007 | 11 Pages |
Abstract
Sesquiterpene lactones are known for their anti-inflammatory activity which has been proven in various assays on DNA, mRNA and protein level. Here we report on the change in the gene expression profile in TNF-α stimulated human 293 cells after treatment with parthenolide using a cDNA microarray analysis. Twenty-one of 7028 genes were found to be up- and 18 down-regulated. They encode for chemoattractants, immune system proteins, glycoproteins, metabolism, serine proteinases, and transcription factors. Confirmatory analyses were carried out using quantitative real-time RT-PCR (TaqMan®). Additional studies with selected genes revealed the concentration-dependent influence of parthenolide on the expression of these genes.
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Authors
M.T. Lindenmeyer, C. Kern, T. Sparna, J. Donauer, J. Wilpert, J. Schwager, D. Porath, C. Kreutz, J. Timmer, I. Merfort,