Article ID Journal Published Year Pages File Type
2554639 Life Sciences 2006 6 Pages PDF
Abstract

This study was designed to compare the effects of oxidized 1-palmitoyl-2-arachidonyl-sn-glycero-3-phosphorylcholine (PAPC) and native PAPC on the inducible nitric oxide synthase (iNOS) in the macrophage cell line RAW 264.7. Macrophages stimulated by bacterial lipopolysaccharide (1 μg/ml) were incubated with increasing amounts of native or oxidized PAPC (oxPAPC, 10–20 μg/ml). Cells incubated with oxPAPC showed a dose-dependent inhibition of inducible nitric oxide synthesis, as well as reduced iNOS protein expression and mRNA levels. Additionally, chromatin immunoprecipitation assay revealed that oxPAPC reduced the interaction of the active NF-κB subunit p65 with the iNOS promoter region when compared to native PAPC.

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