Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
2570968 | Toxicology and Applied Pharmacology | 2007 | 6 Pages |
Abstract
When phenyl benzoate (PB) was used as substrate, about 65% of total activity was resistant to the non-promoter mipafox (up to 0.5Â mM, 20Â min, pHÂ 8.0), that inhibits NTE and other esterases. More than 90% of this resistant activity was sensitive to the classical promoter PMSF (1Â mM, 20Â min, pHÂ 8.0) with an IC50 of about 0.08Â mM (20Â min, pHÂ 8.0). On the contrary, the non-promoter p-toluene sulfonyl fluoride caused only about 10% inhibition at 0.5Â mM. Several esterase inhibitors including, paraoxon, phenyl benzyl carbamate, di-n-butyl dichlorovinyl phosphate and di-isopropyl fluorophosphate, were tested both in vitro and in vivo for inhibition of this PB activity. Mipafox-resistant PMSF-sensitive PB esterase activity(ies) was inhibited by promoters but not by non promoters and neuropathic compounds.
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Authors
A. Moretto, A. Nicolli, M. Lotti,