Towards the synthesis of a highly pure, multiepitopic, mucosal group A streptococcal lipopeptide vaccine

Synthesis of a tri-epitopic Group A streptococcal lipopeptide vaccine was achieved using native chemical ligation. The vaccine was administered intranasally to B10.BR mice, (H-2k) with cholera toxin B-subunit (CTB) or sterile phosphate-buffered saline. Following five boosts, blood was collected for ELISA. Significant IgG antibody titres were observed to the included peptide epitopes following administration with CTB. However, no significant IgG titres were observed in the absence of CTB.