Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
2581526 | Chemico-Biological Interactions | 2009 | 8 Pages |
Abstract
Lanthanides have been reported to induce apoptosis in cancer cell lines. Human cervical cancer cell line HeLa was found to be more sensitive to dicitratolanthanum (III) complex ([LaCit2]3â) than other cancer cell lines. However, the effect and mechanism of dicitratoytterbium (III) complex ([YbCit2]3â) on HeLa cells is unknown. Using biochemical and comparative proteomic analyses, [YbCit2]3â was found to inhibit HeLa cell growth and induce apoptosis. Similar to the effects of [LaCit2]3â, proteomics results from [YbCit2]3â-treated cells revealed profound changes in proteins relating to mitochondria and oxidative stress, suggesting that mitochondrial dysfunction plays a key role in [YbCit2]3â-induced apoptosis. This was confirmed by the decreased mitochondrial transmembrane potential and the increased generation of reactive oxygen species in [YbCit2]3â-treated cells. Western blot analysis showed that [YbCit2]3â-induced apoptosis was accompanied by the activation of caspase-9 and specific proteolytic cleavage of PARP, leading to an increase in the pro-apoptotic protein Bax and a decrease in the anti-apoptotic protein Bcl-2. These results suggest a mitochondrial pathway of cell apoptosis in [YbCit2]3â-treated cells, which will help us understand the molecular mechanisms of lanthanide-induced apoptosis in tumor cells.
Keywords
2′7′-dichlorofluoresceineEF2PHBVDACDTTGAPDHJC-1DCFH-DAPTPdl-dithiothreitolΔΨmPARPSOD13-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazoliumbromideMTTROSPermeability transition poreYtterbiumHeLa cellseukaryotic translation elongation factor 2Quinolinate phosphoribosyltransferaseProhibitinHuman papillomavirusHPVMitochondrial transmembrane potentialProteomicspoly ADP-ribose polymerasevoltage-dependent anion-selective channelglyceraldehyde 3-phosphate dehydrogenaseReactive oxygen species
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Authors
Liming Shen, Qiong Liu, Jiazuan Ni, Guangyan Hong,