Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
2582636 | Chemico-Biological Interactions | 2007 | 12 Pages |
Abstract
The present study is on the growth inhibitory effect of Withania somnifera methanolic leaf extract and its active component, withanolide on HL-60 promyelocytic leukemia cells. The decrease in survival rate of HL-60 cells was noted to be associated with a time dependent decrease in the Bcl-2/Bax ratio, leading to up regulation of Bax. Both the crude leaf extract and the active component activated the apoptotic cascade through the cytochrome c release from mitochondria. The activation of caspase 9, caspase 8 and caspase 3 revealed that caspase was a key mediator in the apoptotic pathway. DNA fragmentation analysis revealed typical ladders as early as 12Â h indicative of caspase 3 role in the apoptotic pathway. Flow cytometry data demonstrated an increase of sub-G1 peak upon treatment by 51% at 24Â h, suggesting the induction of apoptotic cell death in HL-60 cells.
Keywords
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Authors
V. Senthil, S. Ramadevi, V. Venkatakrishnan, P. Giridharan, B.S. Lakshmi, R.A. Vishwakarma, A. Balakrishnan,