| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 2582879 | Environmental Toxicology and Pharmacology | 2016 | 7 Pages |
•Textile effluent was characterized for the presence of different metals.•Textile effluent exhibited strong mutagenic potential in Ames test.•In-vitro comet assay revealed its strong potential for oxidative DNA damage.•Chronic exposure of effluent in rats induced oxidative stress in rat organs.•Vitamin C had ameliorating effects on effluent induced oxidative stress.
Exposure to complex mixtures like textile effluent poses risks to animal and human health such as mutations, genotoxicity and oxidative damage. Aim of the present study was to quantify metals in industrial effluent and to determine its mutagenic, genotoxic and cytotoxic potential and effects on oxidative stress biomarkers in effluent exposed rats. Metal analysis revealed presence of high amounts of zinc, copper, chromium, iron, arsenic and mercury in industrial effluent. Ames test with/without enzyme activation and MTT assay showed strong association of industrial effluent with mutagenicity and cytotoxicity respectively. In-vitro comet assay revealed evidence of high oxidative DNA damage. When Wistar rats were exposed to industrial effluent in different dilutions for 60 days, then activities of total superoxide dismutase and catalase and hydrogen peroxide concentration were found to be significantly lower in kidney, liver and blood/plasma of effluent exposed rats than control. Vitamin C in a dose of 50 mg/kg/day significantly reduced oxidative effects of effluent in rats. On the basis of this study it is concluded that industrial effluent may cause mutagenicity, in-vitro oxidative stress-related DNA damage and cytotoxicity and may be associated with oxidative stress in rats. Vitamin C may have ameliorating effect when exposed to effluent.
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