| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 2583052 | Environmental Toxicology and Pharmacology | 2013 | 8 Pages |
•We assess heavy metal content and genotoxicity in frogs from a coal area.•Contents of heavy metal in frogs from coal area were higher than reference values.•We detected the highest contents of heavy metal in the liver and in the kidneys.•The genotoxicity was significantly higher in specimens from the coal area.
The aims of the study were to determine the heavy metal content in the tissues of Hypsiboas faber from a coal mining area and to compare the DNA damage in the blood cells of these animals with that of animals living in an unpolluted area. The heavy metal content was detected according to the technique of Particle-Induced X-ray Emission (PIXE) and the DNA damage was assessed by the Comet assay. Our results reveal that the specimens of H. faber collected from the coal mining area exhibited elements of order Fe > Cu > Al > Zn > Rb > Mn > Br, independently of the organ. The values of Comet assay parameters (DNA damage index and DNA damage frequency) were significantly higher in specimens collected from the coal mining area than in the reference animals. Our study concludes that the coal mining residues are genotoxic to amphibians and may have adverse effects on soil, water, vegetation and wild animals.
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