| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 2583223 | Environmental Toxicology and Pharmacology | 2013 | 8 Pages |
Production of a sika deer Cu/Zn-SOD was achieved in Pichia pastoris after the reconstituted expression vector pPIC9K was transformed into the strain GS115. By employing Saccharomyces cerevisiae secretion signal peptide (α-factor) under the regulation of the methanol-inducible promoter of the gene of alcohol oxidase 1 (AOX1), sika deer Cu/Zn-SOD with a molecular mass of 16 kDa was expressed while recombinant sika deer Cu/Zn-SOD with an activity of 3500 U/mL was obtained from a 5 L bioreactor. After two successive steps of chromatography on DEAE-650 C and Superdex75, recombinant sika deer Cu/Zn-SOD was obtained with 13.8% yield, 14.5-fold purification, and a specific activity of 3447 U/mg. Its optimum temperature and optimum pH were 40 °C and 7.0, respectively.
Graphical abstractThe expressed SOD has a high thermostability.Figure optionsDownload full-size imageDownload as PowerPoint slideHighlights► A novel Cu/Zn-SOD gene from sika deer was cloned and integrated into P. pastoris. ► The 16-kDa SOD was expressed and secreted in a bioreactor with activity of 3500 U/mL. ► The optimum temperature and pH of the SOD were 40 °C and 7.0, respectively. ► The SOD can be used in investigation on pharmacological effects of antler velvet.
