Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
2583380 | Environmental Toxicology and Pharmacology | 2010 | 7 Pages |
Abstract
This study aimed to evaluate the effect of proguanil, a chemical substance used for treatment and prevention of malaria on viability and DNA integrity in human lymphocytes in vitro. Two different concentrations of proguanil obtained from the plasma concentrations were used: 130Â ng/ml used for prophylactic treatment and 520Â ng/ml used in treatment of malaria. Testing was done with and without metabolic activation. Viability of lymphocytes decreased in time and dose dependent manner. Comet assay parameters showed similar effects, indicating that some damage to DNA molecule can occur. Frequency of sister chromatid exchanges did not show significant deviation from the control samples. As for the proliferation kinetics no significant changes were noticed. Since majority of DNA damaging effect is induced after metabolic activation it is to be concluded that activity of proguanil is dependent upon the active metabolite cycloguanil and that monitoring should be conducted especially among frequent travellers.
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Authors
Goran Gajski, Domagoj Dinter, Vera Garaj-Vrhovac,