| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 2583486 | Environmental Toxicology and Pharmacology | 2012 | 11 Pages |
Organophosphate pesticides can interfere with the serotonergic nervous system and potentially lead to malformations and behavioral abnormalities during early development in sea urchin. To investigate the mechanism by which monocrotophos (MCP) pesticide disrupts the serotonergic nervous system, we evaluated its effects on serotonin metabolism. Fertilized embryos of sea urchin were incubated with 40% MCP pesticide at nominal concentrations of 0.01, 0.10 and 1.00 mg/L, and the effects on tryptophan hydroxylase of Hemicentrotus pulcherrimus (HpTPH), serotonin reuptake transporter (SERT), monoamine oxidase (MAO), and serotonin levels were investigated. The results indicated that MCP pesticide disturbed the baseline pattern of HpTPH and SERT mRNA expression and MAO activity during early development in H. pulcherrimus. When serotonin should be quickly metabolized at 36-hpf stage, HpTPH and SERT expression was decreased and MAO activity was induced by MCP pesticide, leading to the impairment of serotonergic synaptic activity. But when serotonin should be metabolized at low levels during the other six stages, MCP pesticide induced HpTPH and SERT expression, resulting in the improvement of serotonergic synaptic activity. We concluded that this metabolic disturbance is one of the major mechanisms by which MCP pesticides affect the serotonergic nervous system and potentially contribute to various developmental abnormalities.
► In our research, we found that baseline pattern of HpTPH, SERT and MAO, which participate in serotonin metabolism, were disturbed by MCP pesticide during early development in the sea urchin. ► Metabolic disturbance may be one of the major mechanisms by which organophosphate pesticides affect the serotonergic nervous system and potentially contribute to various developmental abnormalities. ► Quantitative real-time RCR was used to investigate neurotoxic effects of organophosphate pesticides on the sea urchin embryos and larvae and to explore the further disturbance mechanisms.
