| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 2583730 | Environmental Toxicology and Pharmacology | 2014 | 14 Pages |
•An idyllic system for monitor onset cellular behavior and photo-oxidative stress.•A sensitive continuous measurement for light-induced cell response of therapeutics.•Measurement of noxious factors during initial and continuous light radiation.•Agmatine and resveratrol to reduce/control the cell damage during radiation.•An effective tool for photo-oxidative stress and photo-damage studies.
A light radiation causes dysfunction and death of retinal cells and leads to degeneration. Present study, investigated the light-induced cell dysfunction, and their activity. Further, the effects of agmatine and resveratrol on light-induced damage and these underlying photo-oxidative and protective mechanisms were monitored by real-time bio-impedance system. After light exposure retinal ganglion cells underwent death in a time dependent manner. During light exposure the cells elevate free radicals and Ca2+, followed by nitric oxide (NO) and tumor necrosis factor-α (TNF-α), which can be facilitated to cell demise. The results revealed that these drugs can control the elevation of free radical, calcium gating, NO level, and increased TNF-α, which could diminish cell photo-damage. In summary, resveratrol helps more to rescue damaged cells compared to agmatine. The proposed system suggested mechanism could meet to identify the photo-toxic effects in retinal cells, and provides high throughput screening for early stages photo-damage.
Graphical abstractFigure optionsDownload full-size imageDownload as PowerPoint slide
