Differential accumulation of BPA in some tissues of offspring of Balb-C mice exposed to different BPA doses

Pregnant adult Balb-C mice were exposed daily to two different doses of Bisphenol A (BPA) by subcutaneous injection beginning on gestational day 1 through the seventh day after delivery. The mothers were sacrificed on postpartum day 21, and the offspring were sacrificed at 3 months of age. Control mice were subjected to the same experimental protocol but received saline injections.The liver, muscles, hindbrain and forebrain of the offspring were dissected and processed using HPLC to assess the level of BPA in the tissues and to determine its dependence on the exposure dose and gender. For comparison, the same tissues were dissected from the mothers and analysed.We report the following results: (1) the level of BPA that accumulated in a given tissue was dependent on the exposure dose; (2) the rank order of BPA accumulation in the various tissues was dependent on the gender of the offspring; (3) the average BPA concentrations in the liver and muscle of the female offspring were higher than in the males; and (4) the average BPA concentration in the central nervous system (i.e., the hindbrain and forebrain) of the male offspring was higher than in the females.

► Pregnant mice were exposed to different BPA doses and its concentration in different organs of the offspring measured. ► The exposure time was from day 1 of gestation until the seventh day after delivery. ► Examined organs were liver, muscle, hindbrain and forebrain. ► BPA concentration was found dependent on exposure dose and on the offspring gender. ► The biological significance of the results is discussed.