| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 2584107 | Environmental Toxicology and Pharmacology | 2012 | 10 Pages |
The study investigated the correlation between infarction areas and behavioural deficits in middle cerebral artery occlusion (MCAO) and photothrombosis stroke models. In the MCAO model, a 0.38 mm-diameter silicone-coated thread was introduced through the left external carotid artery and advanced 18 mm via the internal carotid artery to the origin of middle cerebral artery of male Sprague–Dawley rats weighing 300–350 g. The thread was removed for reperfusion after occlusion for 0.5, 1 or 2 h. In the photothrombosis model, after a midline incision on the scalp, a focused light (10,000 lux, 6 mm-diameter) was delivered 1 mm anterior to the bregma and 3 mm left of the midline for 5, 10 or 20 min. During the first 2 min of irradiation, Rose Bengal dye (30 mg/kg) was injected intravenously. Twenty four hours post-surgery, the animals were subjected to neurological scoring and behavioural performances, and were sacrificed for macroscopic and microscopic examinations of brain injury. Total infarction volumes in the MCAO model rats increased in an occlusion time-dependent manner, while the infarction volumes in photothrombosis model rats plateaued relatively quickly with no time-dependent increase. The MCAO model displayed neurological scores and behavioural deficits that correlated well with infarction volumes, while relatively poor correlation between infarction volume and neurobehavioural abnormalities was evident in the photothrombosis model. The results indicate the suitability of the MCAO model for studies on preventive or therapeutic compounds related to functional recovery, although the photothrombosis model might be useful to generate focused lesions leading to the location-related behavioural changes.
► Two stroke models, middle cerebral artery occlusion (MCAO) and photothrombosis, were compared. ► Brain injury and neurobehavioral deficits according to different occlusion times and irradiation times in each model were examined. ► The relationship between brain injury and behavioural abnormalities were analyzed. ► Behavioural parameters correlate well in MCAO model, but not in photothrombosis model. ► It is suggested that MCAO model is more suitable for the screening of candidate compounds than photothrombosis model.
