| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 2584396 | Environmental Toxicology and Pharmacology | 2008 | 4 Pages |
Abstract
We have identified two most significant biomarker genes, CYP1A1 (69.81 up-regulation) and MT1K (14.66 up-regulation), showing highest overexpression at p-value <0.005. These were selected out of several hundred genes induced in vitro, using PCB exposed human liver (HepG2) cells. Over expression of the CYP1A1 (cytochrome P450) gene was specific to PCB-77 and MT1K (Metallothionein) to PCB-153. Affymetrix oligonucleotide microarrays (mRNA) were used to screen the entire genome of human liver cells in a time-dependent exposure and were further validated by quantitative real-time RT-PCR.
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Authors
Sisir K. Dutta, Somiranjan Ghosh, Supriyo De, Eric P. Hoffman,