| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 2588525 | International Journal of Hygiene and Environmental Health | 2014 | 6 Pages |
PurposeThe aim of this study was to design the optimized laboratory protocol as a tool for human biomonitoring of selected Persistent Organic Pollutants (POPs) in Poland. In this study, we present the method developed for the determination of 29 congeners of polychlorinated dibenzo-p-dioxins (PCDDs), polychlorinated dibenzofurans (PCDFs) and dioxin-like polychlorinated biphenyls (dl-PCBs), as well as individual results of the measurements in 40 human breast milk samples collected in central Poland in 2008–2010.MethodsThe protocol of sample preparation and quantitative analysis of PCDD/Fs and dl-PCBs was optimized for the isotopic dilution method with high resolution gas chromatography/high resolution mass spectrometry HRGC/HRMS. Fat content in the extracts was determined gravimetrically. The results were corrected by fat content in the samples.ResultsThe average sum of PCDD/F and dl-PCBs in the human milk samples from the urban area was 7.429 WHO-TEQ pg/g fat (with the range 0.431–14.27), and in the rural area it was 6.448 pg WHO-TEQ/g fat (0.539–12.61).ConclusionsThe results obtained in this study indicate that the mothers were exposed uniformly to PCDD/Fs and PCBs regardless of location. The significant difference of p < 0.1 between the milk samples from the urban and rural mothers in 2,3,7,8-TCDD; 1,2,37,8,9-HxCDD and 2,3,4,6,7-HxCDF were observed. For the other 14 PCDD/F and 12 dl-PCB congeners, the observed differences were not significant. The total WHO-TEQ values are lower in comparison with the average results in Europe from the fourth round of a WHO-coordinated study.
