Article ID Journal Published Year Pages File Type
2593451 Reproductive Toxicology 2015 20 Pages PDF
Abstract

•The ReProGlo assay is an in vitro tool to predict embryotoxic effects of chemicals.•We extended our test data base from 17 to 80 compounds.•We introduce a new classification scheme including a group of “unspecific positives”.•We compared our results with a structure-based DART prediction approach.•False-positives are very rare in the test and most false-negatives can be explained.

The ReProGlo assay was developed in 2009 to predict embryotoxic potential of drugs and chemicals by use of a stem cell-based in vitro system. It utilizes a luciferase reporter to detect drug-induced alterations in the canonical Wnt/β-catenin signaling pathway, which is involved in regulation of early embryonic development. It allows the simultaneous determination of cell viability and luciferase reporter activity in a high throughput format. The present study was conducted within the framework of the EU ChemScreen-project. It (1) enlarges the original number of test-compounds from 17 to now 80, (2) introduces a new classification scheme and (3) anchors the results against a prediction scheme based on structural features of chemicals. The assay is applicable as stand-alone for priority setting or in a test battery.

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Life Sciences Environmental Science Health, Toxicology and Mutagenesis
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