Apigenin induces apoptosis in Hep G2 cells: Possible role of TNF-α and IFN-γ

Flavonoids are one of the biologically active plant food constituents, possessing potential chemopreventive properties against a wide variety of chronic diseases. Apigenin, a common dietary flavonoid abundantly present in fruits and vegetables is believed to possess preventive and therapeutic potential against various cancers. In the present study, we have evaluated regulation of apoptotic cell death by apigenin (25 and 50 μM) in human hepatoblastoma derived cell line Hep G2. Apigenin-induced programme cell death in terms of TNF-α, IFN-γ release and induction of caspases activity. TNF-α and IFN-γ levels in apigenin-pretreated groups were significantly and dose dependently elevated as compared to the control values (28–39% and 66–85%), (208–336% and 579–1088%), respectively. Treatment of apigenin significantly induced caspase-3, -7, -10 and caspase-9 activity (160–209% and 203–270%) in a dose-dependent manner. The effects on caspases, TNF-α, and IFN-γ processes mediate the plausible mechanism of apoptosis induction of apigenin.