The inhibitory effect of diphenyltin on gap junctional intercellular communication in HEK-293 cells is reduced by thioredoxin reductase 1

Article ID	Journal	Published Year	Pages	File Type
2601482	Toxicology Letters	2008	7 Pages	PDF

Abstract

We found that DPhT inhibits GJIC in HEK-293 cells. The inhibition of GJIC depends on the activation of PKC Î´ and is associated with the induction of Cx43 phosphorylation at Ser262. Moreover, we found that GJIC inhibited by DPhT in HEK-293 cells is fully re-established as a result of TrxR1 overexpression.

Keywords

DMEM phorbol 12-myristate 13-acetate Trx reductase TrxR GJIC Trx PKC 1,1′-dioctadecyl-3,3,3′,3′-tetramethylindocarbocyanine perchlorate DiI PMA Small interfering RNA ROS siRNA gap junctional intercellular communication thioredoxin reductase thioredoxin diphenyltin human embryonic kidney cells HEK-293 cells Dulbecco’s modified eagle’s medium Redox regulation Protein kinase C Reactive oxygen species