Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
2602436 | Toxicology in Vitro | 2015 | 9 Pages |
•The potential roles of lncRNAs in cellular response to radiation-induced DNA damage and oxidative stress were explored.•A set of lncRNAs may response to IR damage in a dose-dependent manner.•LncRNAs serve as novel indicators for radiation toxicity
In the present study, the role of lncRNAs in response to radiation-induced DNA damage and oxidative stress were explored to improve our understanding of the biological pathways activated upon radiation-induced toxicity. The toxicity of X-ray radiation on human bronchial epithelial cell lines (HBE) was determined through a dose-dependent increase in ROS production and γ-H2AX formation and changes to lncRNA expression was observed and quantified using lncRNA-specific microarrays. 115 lncRNAs expression was increased in a dose-dependent manner following X-ray irradiation. Bioinformatic prediction algorithms determined that these lncRNAs significantly affect the p53 signaling pathway, and, more specifically, the BRCA 1 transcription factor and coding genes adjacent to BRCA 1. Our results highlight a previously uncharacterized role for lncRNAs to act via the p53-pathway in response to X-ray-induced DNA damage, and suggest lncRNAs may serve as novel indicators for radiation toxicity.