| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 2602491 | Toxicology in Vitro | 2015 | 9 Pages |
•Solanesol induces HO-1 and Hsp70 expression which activate Nrf2 and HSF1.•Solanesol induces Nrf2 by enhancement of Keap1 interaction with Hsp90.•Solanesol-activated HSF1 is involved in chaperones interaction.•Solanesol inhibits LDH, AST, MDA and ROS level, but increases the GSH level.•Solanesol promotes tBHQ-mediated protective effects.
In present study, we showed that the mRNA and protein levels of HO-1 and Hsp70 in solanesol-treated L02 cells were significantly increased. The induction of the HO-1 by solanesol is majorly achieved via enhancing the nuclear translocation and transactivity of Nrf2 through enhancement of Hsp90–Keap1 interaction, while solanesol-elevated Hsp70 is related with promoting the nuclear translocation of HSF1 through the involvement of chaperones interaction. Furthermore, the induction of HO-1 and Hsp70 by solanesol could protect against ethanol-induced liver injury, including significantly suppressing the elevation of the activities of LDH and AST, attenuating ethanol-induced increase of the MDA, ROS level and decrease of the GSH level. Moreover, solanesol also suppressed ethanol-induced apoptosis of L02 cells by inhibition of nuclear morphological damage, procaspase 3 and cleavage of caspase 3 and PARP, suggesting solanesol may be beneficial against ALD. Solanesol also promoted tBHQ-mediated protective effects. However, treatment cells with SnPP or PES markedly abrogated the protective effects of solanesol on ethanol-induced cell injury. These results strongly suggested that solanesol could protect ethanol-induced L02 cell damage, which might be attributed to the activation of HO-1 and Hsp70.
