| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 2602590 | Toxicology in Vitro | 2012 | 9 Pages |
Lucidone was previously reported to exhibit anti-inflammatory activity in vitro and in vivo. In the present study, we characterized the mechanisms underlying the hepatoprotective effect of lucidone against alcohol-induced oxidative stress in vitro. Human hepatoma (HepG2) cells were pretreated with lucidone (1–10 μg/mL) and then hepatotoxicity was stimulated by the addition ethanol (100 mM). With response to ethanol-challenge, increased amount of alanine aminotransferase (ALT) and aspirate aminotransferase (AST) release were observed, whereas lucidone pretreatment significantly inhibited the leakage of AST and ALT in HepG2 cells without appreciable cytotoxic effects. We also found that lucidone pretreatment significantly decreased ethanol-induced nitric oxide (NO), tumor necrosis factor-α (TNF-α), malondialdehyde (MDA), reactive oxygen species (ROS) and glutathione (GSH) depletion in HepG2 cells. Furthermore, Western blot and quantitative-PCR analyses showed that ethanol-exposure apparently down-regulated endogenous anti-oxidant hemoxygenase-1 (HO-1) expression, whereas pretreatment with lucidone significantly up-regulates HO-1 expression followed by the transcriptional activation of NF-E2 related factor-2 (Nrf-2). Interestingly, the profound up-regulation of HO-1 and Nrf-2 were observed in only ethanol-challenged cells, which evidenced that lucidone-induced induction of HO-/Nrf-2 were specific with oxidative stress. Thus, we concluded that lucidone-mediated up-regulation of phase-II enzymes and HO-1 via Nrf-2 signaling pathway may provide a pivotal mechanism for its hepatoprotective action.
Graphical abstractFigure optionsDownload full-size imageDownload as PowerPoint slideHighlights► Lucidone treatment suppressed EtOH-induced hepatic enzymes and lipid peroxidation. ► Alcohol-exposure elevate NO production in hepatic cells. ► Lucidone treatment up-regulates HO-1 and Nrf-2 expression. ► Lucidone-mediated induction of HO-1 and Nrf2 expression are stress-dependent.
