Article ID Journal Published Year Pages File Type
2602607 Toxicology in Vitro 2011 6 Pages PDF
Abstract

Increases in intracellular Ca2+ during cellular stress often lead to the mitochondrial permeability transition (MPT). We examined changes in fatty acids (FAs) released from isolated renal cortical mitochondria subjected to Ca2+-induced MPT. Exposing mitochondria to Ca2+ stimulated mitochondrial swelling and release of FAs such as arachidonic (20:4) and docosahexenoic acids which increased 71% and 32%, respectively, and linoleic (18:2) which decreased 23% compared to controls. Stearic (18:0), oleic (18:1), and linoleic (18:3) acids were unchanged. To elucidate a mechanism for FA release, mitochondria were pre-treated with bromoenolactone (BEL) to inhibit Ca2+-independent phospholipase A2 gamma activity (iPLA2γ). BEL blocked Ca2+-induced release of arachidonic and behenic (22:0) acids. Finally, four FAs were released in the absence of Ca2+ in a BEL-sensitive manner, including arachidonic and docosatrienoic acids. Thus, extensive FA release occurs during Ca2+-induced MPT, and that mitochondrial iPLA2γ maintains mitochondrial arachidonic acid homeostasis under both basal and Ca2+-induced stress conditions.

► Mitochondrial permeability transition (MPT) involves release of fatty acids. ► Some fatty acids released during MPT involve iPLA2γ. ► iPLA2γ maintains arachidonic acid homeostasis under normal and stressed conditions.

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