Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
2603271 | Toxicology in Vitro | 2009 | 7 Pages |
Abstract
The mycotoxin patulin is a common contaminant of fruit. Here, we demonstrate that patulin reduces the barrier properties of the intestinal cell line, caco-2 by specific effects on tight junction components. Within 5 h of exposure to 100 μM toxin, the transepithelial electrical resistance of caco-2 monolayers was reduced by approximately 95% and the monolayer became more permeable to FITC-labelled dextrans of 4–40 kDa. Immunoblotting revealed occludin proteolysis and a significant reduction in ZO-1 levels. Patulin had no influence on claudin levels but marked changes in their distribution were observed. These data indicate that patulin decreases the barrier properties of caco-2 monolayers by modulation of the tight junction.
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Authors
John McLaughlin, Daniel Lambert, Philip J. Padfield, Julian P.H. Burt, Catherine A. O’Neill,