Article ID Journal Published Year Pages File Type
2754785 Clinical Lymphoma Myeloma and Leukemia 2013 8 Pages PDF
Abstract

BackgroundMycosis fungoides (MF) is a malignant lymphoma characterized by expansion of CD4+ memory T-cell clones. Infiltrating cells express CCR4, which is attracted to CC chemokine ligands 17 and 22 (thymus and activation-regulated chemokine [TARC]/CCL17 and TARC/CCL22). Bath–psoralen plus ultraviolet A (PUVA) is effective against MF. In patients with psoriasis, bath-PUVA induces circulating regulatory T cells (Tregs), which suppress effector T cells. To understand the mechanisms in MF, we analyzed lesion-infiltrating cells before and after bath-PUVA therapy.Patients and MethodsThirteen patients with MF (12 stage IB, 1 stage III; mean age 69.2 years, range 35-87 years; 6 men, 7 women) were recruited.ResultsImmunohistochemical analysis revealed that lesion CCR4-positive (CCR4+) cells and Tregs significantly decreased from 105.1 ± 164.8 cells/10−2 mm2 to 31.4 ± 39.0 cells/10−2 mm2 and from 78.1 ± 67.8 cells/10−2 mm2 to 24.7 ± 25.0 cells/10−2 mm2, respectively. Serum TARC levels significantly correlated with infiltrating CD3+ (r = 0.997), CCR4+ (r = 0.991), and forkhead box P3–positive (Foxp3+) cells (r = 0.843). Circulating Tregs before bath-PUVA therapy were not significantly different from those in healthy volunteers. Bath-PUVA did not significantly change the percentage of circulating Tregs.ConclusionsBath-PUVA decreased CCR4+ cells and Tregs in MF lesions but did not induce circulating Tregs, which might suppress effector T cells. Direct effects through skin lesions might eliminate both pathogenetically relevant cells and Tregs. Systemic immunosuppression was not induced.

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