Article ID Journal Published Year Pages File Type
2778201 Prostaglandins, Leukotrienes and Essential Fatty Acids (PLEFA) 2006 10 Pages PDF
Abstract

The aims of this study were to determine how Prostaglandin E2 (PGE2) locally applied affected the immunodistribution of latent transforming growth factor-beta 1 (TGF-β1), and how the eicosanoid modified TGF-β1 release and TGF-β receptors gene expression in cultured osteoblasts. PGE2 locally delivered on the rat mandible at doses of 0.1 and 0.05 mg/day, but not 0.025 mg/day, over 20 days significantly increased latent TGF-β  1 immunodistribution (P<0.001P<0.001), comparing with a placebo-treated group. Cultured osteoblasts stimulated with 10−5 or 10−7 M PGE2 significantly varied the level of activated TGF-β1 released into supernatants at different experimental periods compared with negative and positive controls. TGF-β   receptor type I gene expression was significantly increased in osteoblasts (P<0.01P<0.01) after 10 days of treatment with 10−5 and 10−7 M PGE2, whereas 10−3 M PGE2 produced the opposite effect. It is concluded that PGE2 may stimulate bone deposition by affecting TGF-β pathway. This effect on the pathway appears to be dose-dependent.

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