| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 2779265 | Bone | 2013 | 9 Pages |
•Preoperative radiation of the hip mediates significantly increased frequencies Tcytotoxic cells, NKT cells and CD25 + CD127 − Treg cells in THA-hematomas.•Significantly higher IL-6, IL-8 and IFNγ concentrations are found in THA-hematomas after radiation.•The concentration of the angiogenic VEGF is significantly suppressed in preoperatively irradiated THA-hematomas.•Preoperative irradiation results in significant changes of immune cell composition and cytokine secretion in THA-hematomas, establishing a proinflammatory milieu.•Increased inflammatory activity and the observed suppression in VEGF secretion are suggested to contribute to the prevention of HO.
Radiation of the hip is an established method to prevent heterotopic ossification (HO) following total hip arthroplasty (THA) but the precise mechanism is unclear. As inflammatory processes are suggested to be involved in the pathogenesis of HO, we hypothesized that the preoperative irradiation impacts local immune components. Therefore, we quantified immune cell populations and cytokines in hematomas resulting from the transection of the femur in two groups of patients receiving THA: patients irradiated preoperatively (THA-X-hematoma: THA-X-H group) in the hip region (7 Gy) in order to prevent HO and patients who were not irradiated (THA-H group) but were postoperatively treated with non-steroidal anti-inflammatory drugs (NSAIDs). Radiation resulted in significantly increased frequencies of T cells, cytotoxic T cells, NKT cells and CD25 + CD127 − Treg cells, whereas the number of naive CD45RA-expressing cytotoxic T cells was reduced. These results indicate differential immune cell activation, corroborated by our findings of significantly higher concentrations of pro-inflammatory cytokines (e.g. IL-6, IFNγ) and chemokines (e.g. MCP-1, RANTES) in the THA-X-H group as compared to THA-H group. In contrast, the concentration of the angiogenic VEGF was significantly suppressed in the THA-X-H group. We conclude that preoperative irradiation results in significant changes in immune cell composition and cytokine secretion in THA-hematomas, establishing a specific – rather proinflammatory – milieu. This increase of inflammatory activity together with the observed suppression in VEGF secretion may contribute to the prevention of HO.
