| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 2785707 | International Journal of Developmental Neuroscience | 2015 | 7 Pages |
•PU leads to increased synaptosomal intracellular calcium ion concentration.•PU leads to increased calpain and caspase-3 activities.•PU leads to cognitive deficits.•PU leads to neuronal death.
Postnatal protein-undernutrition impacts on mental development and cognition in children and can lead to problem with attention and unresponsiveness which compromise children’s ability to learn. These behavioral disorders might be due to alteration in calcium homeostasis as calcium plays critical roles in fundamental functions of neuron. The role of low protein diet as well as Se and Zn supplementation on intracellular calcium concentration ([Ca2+]i), Ca2+-ATPase, Na+-K+-ATPase, calpain and caspase-3 activities from rat cortex and cerebellum were investigated. Well-fed (WF) and low protein diet-fed (LPDF) rats were given diets containing 16% and 5% casein, respectively, for a period of 10 weeks. Then, the rats were supplemented with Se and Zn at a concentration of 0.15 mg L−1 and 227 mg L−1, respectively, in drinking water for 3 weeks. The results obtained from the study showed a significant increase in [Ca2+]i; calpain and caspase-3 activities as well as increase transfer latency in water maze study and reductions in Ca2+-ATPase and Na+-K+-ATPase activities for LPDF rats compared to WF rats. Se and Zn supplementation to LPDF rats reversed the elevation in [Ca2+]i, calpain and caspase-3 activities and restored the cognitive deficits and the activities of Ca2+-ATPase and Na+-K+-ATPase. Conclusively, protein-undernutrition results in the accumulation of synaptosomal calcium and inhibition of calcium transporters presumably via free radical generations and results in cognitive impairment which also probably results from neuronal death in rats through calpain activation and the caspase cascade mechanisms. However, Se and Zn supplementations ameliorated the anomalies observed.
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