| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 2787268 | Journal of Genetics and Genomics | 2015 | 9 Pages |
Abstract
The last couple of years have witnessed an explosion in development of CRISPR-based genome editing technologies in cell lines as well as in model organisms. In this review, we focus on the applications of this popular system in Drosophila. We discuss the effectiveness of the CRISPR/Cas9 systems in terms of delivery, mutagenesis detection, parameters affecting efficiency, and off-target issues, with an emphasis on how to apply this powerful tool to characterize gene functions.
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Authors
Jiang Xu, Xingjie Ren, Jin Sun, Xia Wang, Huan-Huan Qiao, Bo-Wen Xu, Lu-Ping Liu, Jian-Quan Ni,